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Clone: 2F6 克隆:2F6 |
Volume: 100 μl (0.5 μg/μl) |
Specificity: Recognizes domain I of JFH-1 NS5A (genotype 2a) |
Cross-reactivity: Cross-reactive against genotype 1a |
Form: Protein G purified 形式:G蛋白纯化 |
Origin: Mouse 来源:小鼠 |
Isotype: IgG2a 同种型:IgG2a |
Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3) 缓冲液/成分:1X PBS pH 7.2;0.1% 叠氮化钠 (NaN3) |
Storage: Short Term (<6 months): 4℃. Long Term (up to 1 year): -20℃ (avoid repeated freeze/thaw) 储存:短期(< 6个月):4℃ 长期(可达1年):-20℃(避免反复冻融) |
Applications: Ideal for immunofluorescence, can also be used for Western Blot at low dilutions. Co-Immunoprecipitation 应用:免疫荧光(immunofluorescence)最理想,低浓度下也可用于免疫印迹(Western Blot).适用于免疫共沉淀实验(Co-IP) |
Working Dilutions: IF-1:2,000-1:6,000, Western Blot- 1:500 |
References:
参考文献:
(1) Backes et al. J Virol. 2010 Jun;84(11):5775-89
Cell lysate run on 12% SDS-PAGE under non-reducing conditions and transferred to PVDF. Primary antibody incubation performed overnight at 4℃ .
细胞裂解物在12% SDS-PAGE(非还原条件)电泳并转膜(PVDF)。一抗4℃过夜孵育。
Item #: HCV-2F6
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