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Clone: 7B5 克隆:7B5 |
Volume: 100 μl (0.5 μg/μl) |
Specificity: Recognizes domain I of JFH-1 NS5A (genotype 2a) |
Form: Protein G purified 形式:G蛋白纯化 |
Origin: Mouse 来源:小鼠 |
Isotype: IgG2b 同种型:IgG2b |
Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3) 缓冲液/成分:1X PBS pH 7.2;0.1% 叠氮化钠 (NaN3) |
Storage: Short Term (<6 months): 4℃. Long Term (up to 1 year): -20℃ (avoid repeated freeze/thaw) 储存:短期(< 6个月):4℃ 长期(可达1年):-20℃(避免反复冻融) |
Applications: Ideal for Western Blot assay 应用:最适用于免疫印迹 |
Working Dilutions: Western Blot- 1:1000-1:10,000 工作稀释度:Western Blot- 1:1000-1:10,000 |
Cell lysate run on 12% SDS-PAGE and transferred to PVDF. Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable).
细胞裂解物在12% SDS-PAGE电泳并转膜(PVDF)。一抗室温孵育1小时(过夜孵育也可)。
REFERENCES:
参考文献:
Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS5A RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.
Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.
Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation. PLoS Pathog 2012; 8 (4).
Grisé H, Frausto S, Logan T, Tang H. A conserved tandem cyclophilin-binding site in hepatitis C virus nonstructural protein 5A regulates Alisporivir susceptibility. J Virol. 2012 May;86(9):4811-22
Item #: HCV-7B5
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