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贝若凡生物科技(合肥)有限公司

检测认证人脉交流通讯录

Anti-HCV NS5A antibody[7B5]

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  • 品  牌:
  • BioFront
  • 主要规格:
  • 100μl/支
  • 用  途:
  • WB
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      Clone: 7B5

      克隆:7B5

      Volume:  100 μl (0.5 μg/μl)
      体积:100 μl (0.5 μg/μl)

      Specificity: Recognizes domain I of JFH-1 NS5A (genotype 2a)
      特异性:识别JFH-1 NS5A (基因型2a)的domain I

      Form: Protein G purified

      形式:G蛋白纯化

      Origin: Mouse

      来源:小鼠

      Isotype: IgG2b

      同种型:IgG2b

      Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)

      缓冲液/成分:1X PBS pH 7.2;0.1% 叠氮化钠 (NaN3)

      Storage: Short Term (<6 months): 4℃.  Long Term (up to 1 year): -20℃ (avoid repeated freeze/thaw)

      储存:短期(< 6个月):4℃ 长期(可达1年):-20℃(避免反复冻融)

      Applications: Ideal for Western Blot assay

      应用:最适用于免疫印迹

      Working Dilutions: Western Blot- 1:1000-1:10,000

      工作稀释度:Western Blot- 1:1000-1:10,000

       

      Cell lysate run on 12% SDS-PAGE and transferred to PVDF.  Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable).

      细胞裂解物在12% SDS-PAGE电泳并转膜(PVDF)。一抗室温孵育1小时(过夜孵育也可)。

       

      REFERENCES:

      参考文献:


      Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS5A RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.


      Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.


      Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation.  PLoS Pathog 2012; 8 (4).


      Grisé H, Frausto S, Logan T, Tang H. A conserved tandem cyclophilin-binding site in hepatitis C virus nonstructural protein 5A regulates Alisporivir susceptibility. J Virol. 2012 May;86(9):4811-22


       

      • Item #: HCV-7B5

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