THE MONOTRACE ADVANTAGE（优势）

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1、Target a major allergen resistant to food processing effects

2、Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility 

  of‘false positive’results

3、Perform well in a wide variety of food products with negligible food matrix interference

BioFront Technologies开发了第一条完全以单克隆抗体为基础的检测试剂盒产品（MonoTrace ELISAs），检测各种食物基质中的致敏蛋白污染。MonoTrace ELISAs综合使用一系列精准确定的经过严格筛选过程后仔细挑选的单克隆抗体来实现：

1、分析耐食物加工的主要过敏原

2、特异性识别目标成分，从而消除交叉反应，以及减少“假阳性”结果的可能性

3、在各种食品（食品基质干扰可以忽略不计）的检测中表现优异

EGG ASSAY PERFORMANCE

鸡蛋（卵黏蛋白）分析试剂盒性能

Overall Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 48 & 96

Limit of Detection (whole egg powder): 0.2 ppm

Range of Quantification (whole egg powder): 1 to 40 ppm

Specificity: Egg ovomucoid (Egg white)

实验总时间：现在抽提后仅需30分钟！

孵育时间：抽提，样品孵育，二抗孵育，底物孵育每步仅需10分钟。

每一试剂盒测试数量：48或96

*检测限（全蛋粉）：0.2 ppm

*定量范围：1至40 ppm

特异性：蛋卵类黏蛋白（蛋清）

*=参数的计算根据10次代表性的数据得出，然而，使用者应当查阅附随的分析证书中的具体批次数值。

CROSS-REACTIVITY 交叉反应

At concentrations as high as 100,000 ppm, no cross-reactivity was observed in a large panel of assayed samples, including: wheat, rice, corn, buckwheat, Quinoa, white beans, kidney beans, pinto beans, lima beans, green peas, chick peas, lentils, soy bean flour, soy milk, peanuts, tree nuts, sesame seeds, sunflower seeds, poppy seeds, pumpkin seeds, mustard seed, celery flour, chicken meat, turkey meat.

在浓度高达100,000 ppm时，对一份大型分析样品和基质中没有观察到交叉反应，包括：小麦，水稻，玉米，荞麦，藜麦，白豆，芸豆，斑豆，利马豆，荷兰豆，嘴豆，扁豆，大豆粉，豆奶，花生，树坚果，芝麻籽，葵花籽，罂粟籽，南瓜籽，芥菜籽，芹菜粉，鸡肉，火鸡肉。

RECOVERY 回收

Recovery of spiked whole egg powder samples at various concentrations was efficient (>90%) when the following food matrices were assayed:  flours (wheat, rice, corn), salad dressing, wine, ice cream

当分析以下食物基质时，不同浓度全蛋粉样品的加标回收率很高（>90%）：面粉（小麦，水稻，玉米），沙拉酱，葡萄酒，冰激凌