丙型肝炎病毒NS3抗原检测试剂盒（HCV NS3 ELISA Kit）

The BioFront Technologies hepatitis C virus (HCV) non-structural protein 3 (NS3) enzyme-linked immunosorbent assay (ELISA) is a kit that allows for the quantitative detection of HCV NS3 within cellular lysates. The NS3 ELISA is suitable for testing drug-treated cell lines, knock-down or knockout cell lines for the presence of HCV replication, or for evaluating HCV infections in vitro. This   assay provides a high throughput alternative to conventional viral detection   methods such as reverse transcription polymerase chain reaction (RT-PCR) and western blotting. It also enables facile and quantitative measure of HCV   replication without the need to incorporate reporter genes into HCV genomes.

*This ELISA kit is NOT intended to be used for detecting the presence of viral particles in patient sera or cell culture supernatant.

BioFront Technologies的丙肝病毒（HCV）非结构蛋白3（NS3）酶联免疫吸附试验（ELISA）试剂盒能够定量检测细胞裂解液中的HCV NS3。NS3 ELISA试剂盒适合检测药物治疗细胞系、抑制或敲除细胞系中HCV复制的存在与否，或用于体外评价HCV感染。本试剂盒采用的方法为传统的病毒检测方法（如逆转录聚合酶链式反应（RT-PCR）、免疫印迹（western blotting））提供了高通量的替代选择。本方法操作简便，并可定量检测HCV复制，无需将报告基因引入HCV基因组。

*本ELISA试剂盒不可用于检测病人血清或细胞培养上清中病毒颗粒的存在与否。

Specificity:   Antibodies  employed recognize conserved epitopes on the NS3 protein from all genotypes; Detection limits experimentally determined for genotypes 1a, 1b and 2a NS3   protein

特异性：抗体应用于识别所有基因型的NS3蛋白上的保守表位；适用于基因型1a，1b，以及2a 的 NS3蛋白

Form: Monoclonal   antibody coated 48&96-well plate comes in convenient removable strip   format (6&12 X 8-well strips)

形式：包被单抗的48&96孔板，形成可方便拆卸的测试条规格（6&12   X 8孔-测试条）

Components:  48&96-well plate, NS3 standard,  10X cell lysis buffer, 5X wash buffer, HRP-conjugated anti-NS3 detection antibody, 5X conjugate dilution buffer, high sensitive HRP-substrate   solution, HRP quench solution

组成：96孔板，NS3标准品，10X细胞裂解缓冲液，5X洗涤缓冲液，HRP偶联的抗NS3检测抗体，5X耦合物稀释缓冲液，高敏感性的HRP底物溶液，HRP淬灭溶液

Storage/Shelf   life: Each plate is packed in a vacuum-sealed Mylar(™)  pouch with desiccant and oxygen absorbing packets to extend the shelf life of the product to 6 months from the date of manufacture, if kept unopened and   stored at 4℃.

存储/保质期：每一个板包装在真空包装聚酯薄膜(™)袋内，内附干燥剂和氧气吸收剂小包。如果不开封并在4℃存储，能够延长产品的保质期至6个月（从生产日期起）。

检测限（LOD）

1   ng/孔

15   pmol/孔

定量限（LOQ）

120   ng/孔

600   pmol /孔

线性范围

1-120   ng/孔

15-600   pmol /孔

刺激指数（S1200/S0）

~3.00   / ~0.055

＞30

标准最大OD值/分析背景

敏感度（S9/S0）

~0.105   / ~0.055

＞1.9

标准最小OD值/分析背景

Figure   1：Example of NS3 ELISA   standard curve data. An uninfected cell lysate was prepared with 1e6 cells   and 0.5 mL cell lysis buffer. NS3h standard was serially diluted using 2-fold   dilutions and spiked into the cell lysate. Values were blanked using 0 ng/mL   cell lysate. The linear range extends from 600 to 9.4 ng/mL.

图1：NS3   ELISA标准曲线一例。未感染细胞裂解物由1×106细胞，0.5 mL细胞裂解液制备。NS3h标准品用2倍的连续稀释法稀释并作为加标物加入细胞裂解液。所有数值均扣除0 ng/mL 细胞裂解物的背景值。线性范围为600至9.4   ng/mL。