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正常人主要细胞溶解产物

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    • 正常人主要细胞溶解产物 Preparation:Cell lysate was prepared in modified RIPA buffer (50mMTris, pH7.2, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% NP-40 , 1mM Na3VO4, 5mM NaF, 400uM PMSF, 1ug/ml of pepstatin A, 5ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was diluted and boiled for 5 min with nonreducing sample buffer (50 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue) containing 5% beta-mercaptoethanol. Concentration:2 mg/ml Storage: Stable for 6 months at -20oC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing. Application: Cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Cat.No. Product Name Size Price 1106 Human brain vascular smooth muscle cell lysate 100 µg $330 人脑血管平滑肌细胞 1206 Human brain vascular pericyte lysate 100 µg $330 人脑血管周边细胞 1406 Human meningeal cell lysate 100 µg $330 人脑膜细胞 1526 Human neuron lysate 100 µg $330 人神经元细胞 1606 Human oligodendrocyte precursor cell lysate 100 µg $330 人少突先驱胶质细胞 1716 Human perineurial cell lysate 100 µg $330 人周神经细胞 1806 Human astrocyte lysate 100 µg $330 人星形胶质细胞 1906 Human microglia lysate 100 µg $330 人小胶质细胞 2006 Human dermal microvascular endothelial cell lysate 100 µg $330 人皮肤微血管内皮细胞 2016 Human dermal vascular smooth muscle cell lysate 100 µg $330 人皮肤血管平滑肌细胞 2106 Human dermal kerotinocyte lysate 100 µg $330 人皮肤角质细胞 2206 Human dermal melanocyte lysate 100 µg $330 人皮肤黑素细胞 2306 Human dermal fibroblast lysate 100 µg $330 人皮肤纤维原细胞 2506 Human lymphatic endothelial cell lysate 100 µg $330 人淋巴内皮细胞 2536 Human lymphatic fibroblast lysate 100 µg $330 人淋巴纤维原细胞 2626 Human gingival fibroblast lysate 100 µg $330 人齿龈纤维原细胞 2636 Human peridontal ligmant firbroblast lysate 100 µg $330 人齿根膜韧带纤维原细胞 2716 Human esophageal smooth muscle cell lysate 100µg $330 人食管平滑肌细胞 2906 Human intestinal microvascular endothelial cell lysate 100 µg $330 人肠微血管内皮细胞 2916 Human intestinal smooth muscle cell lysate 100 µg $330 人肠平滑肌细胞 3006 Human pulmonary microvascular endothelial cell lysate 100 µg $330 人肺微血管内皮细胞 3106 Human pumonary artery endothelial cell lysate 100 µg $330 人肺动脉内皮细胞 3116 Human pulmonary artery smooth muscle cell lysate 100 µg $330 人肺动脉平滑肌细胞 3126 Human pulmonary artery fibroblast lysate 100 µg $330 人肺动脉纤维原细胞 3206 Human pulmonary alveolar epithelial cell lysate 100 µg $330 人肺齿槽上皮细胞 3306 Human pulmonary fibroblast lysate 100 µg $330 人肺纤维原细胞 3406 Human bronchial smooth muscle cell lysate 100 µg $330 人支气管平滑肌细胞 3416 Human tracheal smooth muscle cell lysate 100 µg $330 人气管平滑肌细胞 3506 Human skeletal muscle cell lysate 100 µg $330 人骨骼肌细胞 4006 Human renal glomerular endothelial cell lysate 100 µg $330 人肾小球内皮细胞 4106 Human renal proximal tubular epithelial cell lysate 100 µg $330 人肾最接近管状上皮细胞 4116 Human renal cortical epithelial cell lysate 100 µg $330 人肾皮层上皮细胞 4126 Human renal epithelial cell lysate 100 µg $330 人肾上皮细胞 4206 Human renal mesangial cell lysate 100 µg $330 人肾系膜细胞 4306 Human bladder microvascular endothelial cell lysate 100 µg $330 人膀胱微血管内皮细胞 4316 Human bladder smooth muscle cell lysate 100 µg $330 人膀胱平滑肌细胞 4336 Human bladder stromal fibroblast lysate 100 µg $330 人膀胱基质纤维原细胞 4606 Human calvarial osteoblast lysate 100 µg $330 人颅盖造骨细胞 5006 Human hepatic sinusoidal endothelial cell lysate 100 µg $330 人肝窦状腺内皮细胞 5106 Human intraheptic biliary epithelial cell lysate 100 µg $330 人肝胆上皮细胞 5206 Human hepatocyte lysate 100 µg $330 人肝细胞 6006 Human cardiac microvascular endothelial cell lysate 100 µg $330 人心脏微血管内皮细胞 6106 Human aortic endothelial cell lysate 100 µg $330 人大动脉内皮细胞 6116 Human aortic smooth muscle cell lysate 100 µg $330 人大动脉平滑肌细胞 6206 Human cardiac myocyte lysate 100 µg $330 人心肌细胞 6526 Human keratocyte lysate 100 µg $330 人角膜细胞 6546 Human retinal pigment epithelial cell lysate 100 µg $330 人视网膜色素上皮细胞 6556 Human lens epithelial cell lysate 100 µg $330 人视镜上皮细胞 6566 Human iris pigment epithelial cell lysate 100 µg $330 人虹膜色素上皮细胞 6586 Human non-pigment ciliary epithelial cell lysate 100 µg $330 人非色素睫毛上皮细胞 7106 Human amniotic epithelial cell lysate 100 µg $330 人羊膜上皮细胞 7126 Human villous trophoblast lysate 100 µg $330 人长茸毛滋养层 7136 Human villous mesenchymal fibroblast lysate 100 µg $330 人长茸毛间叶纤维原细胞 7216 Human preadipocyte lysate 100 µg $330 人前脂肪细胞 8006 Human umbilical vein endothelial cell lysate 100 µg $330 人脐静脉内皮细胞 8016 Human umbilical artery endothelial cell lysate 100 µg $330 人脐动脉内皮细胞 8026 Human umbilical vein smooth muscle cell lysate 100 µg $330 人脐静脉平滑肌细胞 8036 Human umbilical artery smooth muscle cell lysate 100 µg $330 人脐动脉平滑肌细胞

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