微核(micronucleus,简称MCN)是真核生物细胞中的一种异常结构,是由有丝分裂后期丧失着丝粒的染色体片断游离于细胞质中产生的。细胞受遗传毒物作用形成微核,以检测细胞中微核的形成来检测物质遗传毒性的试验称为微核试验。目前,微核试验已被许多国家及机构纳入标准检测试验,用于评价新药、食品添加剂、农药、化妆品、工业化学品、环境污染物等遗传毒性的检测、安全性评价和遗传损害的检测。
体外流式微核分析,让该过程变得更加简单、快捷、可靠,并可获得更丰富的信息。
流式细胞术是检测微核的新兴技术平台。相比于传统的显微镜下计数微核的方法,流式细胞技术的检测优势在于:高速高通量,多细胞多参数,高特异性,多信息。
In Vitro MicroFlow Kits 流式细胞术体外微核检测试剂
试剂规格:
IN VITRO-250/50:Analyze 250 samples in 96-well plates or analyze 50 samples in larger format plates or flasks.
IN VITRO-1,000/200:Analyze 1,000 samples in 96-well plates or analyze 200 samples in larger format plates or flasks.
IN VITRO-2,000/400:Analyze 2,000 samples in 96-well plates or analyze 400 samples in larger format plates or flasks.
试剂运输条件:
Ambient
试剂盒存储条件:
Freezer (-20 °C)
Refrigerator (4 °C)
Ambient
试剂盒组成:
Incomplete Lysis Solution 1: Lyses cells after staining
Incomplete Lysis Solution 2: Lyses cells after staining
Nucleic Acid Dye A: Stains the DNA of cells undergoing apoptosis or necrosis
Nucleic Acid Dye B: Stains nuclei and micronuclei
RNase Solution: Removes RNA so Nucleic Acid Dye B stains only nuclei and micronuclei
10x Buffer: Used in the lysis solutions
相关材料的要求:
Cell line and cell culture materials
Freezer (-20 °C) and refrigerator (4 °C)
Centrifuge and other general lab supplies
Flow cytometer capable of 488 nm excitation
Light source (fluorescent preferred)
If using 96-well plates, an 8-channel bridge and aspirator manifold (available from V&P Scientific)
Litron Laboratories 相关产品:
1、Litron Laboratories 流式细胞术检测试剂 产品目录
2、In Vitro MicroFlow Kits 流式细胞术体外微核检测试剂
3、In Vivo MicroFlow Kits 流式细胞术体内微核检测试剂
4、In Vivo MutaFlow Kits 流式细胞术大鼠体内Pig-a基因突变检测试剂
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