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供GenomONE™ - CF EX Sendai virus (HVJ) Envelope Cell Fusion Kit 欣博盛供
货号：ISK-CF-001-EX
名称：GenomONE™ - CF EX Sendai virus (HVJ) Envelope Cell Fusion Kit
规格：1 SET
产品描述：
GenomONE™-CF EX是一种细胞融合套件，由hvj封套（hvj-e）和特殊缓冲液组成。它可用于粘附细胞和漂浮细胞。用这个试剂盒30分钟内可以融合相同或不同类型的细胞。
HVJ Envelope是仙台virus完全灭活后制备的纯化产物。它是一种仅保留仙台virus包膜蛋白细胞膜融合能力的囊泡。
众所周知，仙台virus外膜中的hn蛋白识别细胞膜上的受体（在糖链末端具有唾液酸），并对其进行吸附，从而诱导由F蛋白（包膜的另一组分）介导的膜融合。hvj-e所含仙台virus的基因组RNA已完全失活，在人类和实验动物中既没有infect性也没有增殖潜力。hvj-e可在普通实验室安全使用，无需任何特殊操作或设施。
特点与优势：
Cmparison with PEG method in hybridoma preparation
Hybridoma
growth supplement
Hybridoma
positive rate
antibody-production
positive rate
GenomONE - CF EX
-
38/96 (40%)
9/96 (9%)
+
96/96 (十分)
96/96 (十分)
PEG1500
-
3/96 (3%)
1/96 (1%)
+
36/96 (38%)
9/96 (9%)
GenomONE - CF EX produces more antibody secreting hybridomas than PEG. (following cell fusion, cells were grown in media containing hybridoma growth supplement as indicated).
Rat MSC cells (rat bone marrow-derived mesenchymal stem cells) labeled with red fluorescence*1 were combined with rat primary cardiac myocytes labeled with green fluorescence*2 (each 2 x 105 cells) in 50 µL of a buffer for cell fusion. The mixture was incubated on ice for 5 minutes and then at 37°C for 15 minutes. As a result, fused cells (yellow) were formed (GenomONE-CF suspension method). Fused cells adhering to the plate were also observed after 1-2 days of culturing. In the PEG-treated group, high cytotoxicity appeared immediately after cell fusion, reducing the number of fused cells obtained.
(*1)Labeled with PKH26 Red Fluorescent Cell Linker Kit (Sigma)
(*2)Labeled with PKH67 Green Fluorescent Cell Linker Kit (Sigma)应用：
1.Hybridoma preparation
Cell fusion involving PEG and electrofusion Preparation of monoclonal antibodies Fusion of antigen-sensitized B cells and myeloma cells
2. Fusion of suspension cells
3. Fusion of adherent cell and suspension cell
4. Application in premature chromosome condensation (PCC)
Detection of chromosomal damage and repair by fusion of different types of cells in different cell phases
5. Research on regenerative medicine and cytotherapy
Fusion of somatic cells and stem cells, etc.
6. Research on embryonic development/differentiation
Nuclear transfer (nuclear replacement)
试剂盒组成：
GenomONE™ - CF EX Kit Contents
(for 10 hybridoma fusion experiments)
Freeze-dried Sendai virus envelope (HVJ-E)
0.26 mL
equivalent
Sendai virus envelope (HVJ-E) suspension buffer
0.5 mL
20x Cell Fusion Buffer
10 mL
实验方法：
关于Cosmo Bio
Cosmo Bio公司成立于1983年，是日本领先的生命科学科研试剂供应商，为全球各地的实验室、研究所和生命科学教育与检测机构等提供领先技术的产品和技术信息。其丰富的产品种类、快捷的物流、详尽的产品资料、专业的客户支持与服务，支持和激励着日本生命科学的发展。
Cosmo Bio产品种类众多，涵盖抗原、抗体、试剂盒、分子化合物、细胞培养相关试剂、蛋白合成与标记试剂、基因工程相关产品等等。
畅销产品：
▷ l Labiase™ Bacterial Cell Lytic Enzymes 乳酸菌细胞壁消化酶
可有效溶解大量乳酸菌的细胞壁，用于研究乳酸菌细胞壁的结构、乳酸菌质粒DNA，胞内酶和原生质体的制备。
活性：10 units / g (β-N-Acetyl-D-glucosaminidase)
产品货号
产品名称
规格
OZK-OZ-30EX
Labiase™ Bacterial Cell Lytic Enzymes
500mg
▷ l CARD HyperOva 小鼠超数排卵诱导剂
传统的超排卵方法（PMSG-hCG法），平均每只小鼠（C57BL/6J）只能获得大约20个卵子，使用CARD HyperOva产品，可以获得3~4倍的卵子。
相关产品：
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产品名称
规格
KYD-010-06-EX
CARD HyperOva
0.6 ML
KYD-010-EX
CARD HyperOva
1 ML
KYD-010-06-EX-X5
CARD HyperOva
5*0.6 ML
KYD-010-EX-X5
CARD HyperOva
5*1 ML
▷l FERTIUP® Mouse Sperm Cryoprotectant: CPA
用于稳定精子超低温保存的效果，改善实验小鼠体外受精效率。
精子冷冻保存是实验室小鼠品系保存的经济实用的方法。FERTIUP@和CARD MEDIUM@产品是用于小鼠精子冷冻保存、精子孵育及体外受精的高性能培养基。当FERTIUP@和CARD MEDIUM@联合使用时，可使冻融的小鼠精子保持稳定的高受精率，在您需要的时候可以繁殖小鼠。相比传统方法，FERTIUP精子冻存液、预孵化培养基可significan改善C57BL/6 和 129小鼠品系冻融精子的受精率10%~30%。此外，新的CARD MEDIUM@将繁殖率提高到了80% 以上。FERTIUP@和CARD MEDIUM@不存在任何的生殖毒性，为安全可靠的保存您珍贵的小鼠品系提供了强有力的支持。
相关产品：
产品货号
产品名称
规格
KYD-001-05-EX
FERTIUPⓇMouse Sperm Cryoprotectant: CPA
0.5
KYD-001-EX
FERTIUPⓇCryoprotectant: CPA
1 ML
KYD-001-05-EX-X5
FERTIUPⓇCryoprotectant: CPA
5*0.5 ML
KYD-001-EX-X5
FERTIUPⓇCryoprotectant: CPA
5*1 ML
KYD-005-EX
FERTIUPⓇPM 0.5ML-CARD MEDIUM((R)) set
1 SET
KYD-004-EX
FERTIUPⓇPM 1ML-CARD MEDIUM((R)) set
1 SET
KYD-002-05-EX
FERTIUPⓇPreincubation Medium: PM
0.5 ML
KYD-002-EX
FERTIUPⓇPreincubation Medium: PM
1 ML
KYD-002-05-EX-X5
FERTIUPⓇPreincubation Medium: PM
5*0.5 ML
KYD-002-EX-X5
FERTIUPⓇPreincubation Medium: PM
5*1 ML
KYD-003-EX
CARD MEDIUM((R))
1 KIT
KYD-004-EX
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1 SET
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